![]()
Factory direct 5050 yellow light LED - high quality, high brightness, perfect for industrial and commercial lighting applications.
![]()
High frequency needle 10445 with line - designed for precision testing, capable of handling up to 3A current, ideal for electronic and electrical systems.
High-performance liquid chromatography (HPLC) is a widely used analytical technique in laboratories for separating, identifying, and quantifying compounds in a mixture. However, like any complex instrument, it can experience various issues that affect its performance. Diagnosing and resolving these problems requires a systematic approach.
Faults in an HPLC system can be identified from multiple perspectives. First, by monitoring the operation of key components such as pressure and flow rate, which are critical indicators of system health. A sudden drop or rise in pressure, or an unstable reading, may signal a blockage, leak, or faulty pump. Second, abnormalities in the chromatogram—such as peak fronting, tailing, broadening, or splitting—can provide clues about column performance, sample preparation, or mobile phase issues. Third, analyzing the data results—whether quantitative or qualitative—can reveal inconsistencies that point to underlying problems in the system.
### I. Pressure-Related Issues
1. **No Pressure**: This could indicate a failed pump, broken tubing, or a disconnected system.
2. **High Pressure**: Often caused by clogged columns, filters, or restrictions in the flow path.
3. **Low Pressure**: May result from leaks, air bubbles, or incorrect settings.
4. **Unsteady Pressure**: Could be due to pump malfunction, improper solvent mixing, or temperature fluctuations.
### II. Chromatogram Abnormalities
1. **Peak Fronting**: Suggests poor column efficiency or excessive sample loading.
2. **Peak Tailing**: Usually indicates column degradation or contamination.
3. **Peak Broadening**: May be caused by slow flow rates, column overloading, or temperature instability.
4. **Peak Bifurcation**: Indicates possible column damage or mobile phase issues.
5. **No Peak**: Could be due to detector failure, incorrect injection, or non-detectable compounds.
6. **Negative Peak**: Might occur if the baseline is not properly set or there's a detector issue.
7. **Ghost Peak**: Often results from carryover or contamination in the system.
8. **Retention Time Changes**: Can be caused by temperature variations, mobile phase changes, or column aging.
9. **Retention Time Keeps Changing**: Suggests inconsistent system conditions or unstable flow.
10. **Baseline Drift**: Usually related to temperature, solvent composition, or detector issues.
11. **Irregular Baseline Noise**: May come from electrical interference or impurities in the mobile phase.
12. **Regular Baseline Noise**: Often linked to pump pulsation or mechanical vibrations.
### III. Data Analysis
1. **Quantitative Results**: Inaccurate readings may point to calibration errors, detector drift, or improper sampling.
2. **Qualitative Results**: Misidentification of peaks could stem from column performance, solvent purity, or incorrect method parameters.
**Tester: Your one-stop after-sales service platform for the testing industry.**
We offer comprehensive support for all your laboratory equipment needs:
- **Instrument Repair**: Professional maintenance and repair services for a wide range of instruments.
- **Used Equipment**: Reliable second-hand instruments at competitive prices.
- **Equipment Rental**: Flexible leasing options for GC, IC, and more, including free maintenance and trade-in programs.
- **Consumables & Spare Parts**: High-quality chromatography consumables, lab supplies, and replacement parts available for purchase or auction.
Whether you're looking for maintenance, repairs, rentals, or consumables, Tester has you covered. Visit us today at [http://](http://) for expert assistance and solutions tailored to your lab’s needs.
Line Interactive UPS
Back-Up UPS,Avr Boost And Buck,Battery Low Voltage Protection,Dual Voltage Power Supply
Shenzhen Unitronic Power System Co., Ltd , https://www.unitronicpower.com